Scripted Stereotypes In Reality TV

Diversity, or lack thereof, has always been an issue in both television and film for years. But another great issue that ties in with the lack of diversity is misrepresentation, or a substantial presence of stereotypes in media. While stereotypes often are commonplace in scripted television and film, the possibility of stereotypes appearing in a program that claims to be based on reality seems unfitting. It is commonly known that reality television is not completely “unscripted” and is actually molded by producers and editors. While reality television should not consist of stereotypes, they have curiously made their way onto the screen and into our homes. Through content analysis this thesis focuses on Latina/Hispanic-American and Asian-American contestants on ABCs’ The Bachelor and whether they present stereotypes typically found in scripted programming

Mapping of RNA- temperature-sensitive mutants of Sindbis virus: assignment of complementation groups A, B, and G to nonstructural proteins

Four complementation groups of temperature-sensitive (ts) mutants of Sindbis virus that fail to make RNA at the nonpermissive temperature are known, and we have previously shown that group F mutants have defects in nsP4. Here we map representatives of groups A, B, and G. Restriction fragments from a full-length clone of Sindbis virus, Toto1101, were replaced with the corresponding fragments from the various mutants. These hybrid plasmids were transcribed in vitro by SP6 RNA polymerase to produce infectious RNA transcripts, and the virus recovered was tested for temperature sensitivity. After each lesion was mapped to a specific region, cDNA clones of both mutants and revertants were sequenced in order to determine the precise nucleotide change responsible for each mutation. Synthesis of viral RNA and complementation by rescued mutants were also examined in order to study the phenotype of each mutation in a uniform genetic background. The single mutant of group B, ts11, had a defect in nsP1 (Ala-348 to Thr). All of the group A and group G mutants examined had lesions in nsP2 (Ala-517 to Thr in ts17, Cys-304 to Tyr in ts21, and Gly-736 to Ser in ts24 for three group A mutants, and Phe-509 to Leu in ts18 and Asp-522 to Asn in ts7 for two group G mutants). In addition, ts7 had a change in nsP3 (Phe-312 to Ser) which also rendered the virus temperature sensitive and RNA-. Thus, changes in any of the four nonstructural proteins can lead to failure to synthesize RNA at a nonpermissive temperature, indicating that all four are involved in RNA synthesis. From the results presented here and from previous results, several of the activities of the nonstructural proteins can be deduced. It appears that nsP1 may be involved in the initiation of minus-strand RNA synthesis. nsP2 appears to be involved in the initiation of 26S RNA synthesis, and in addition it appears to be a protease that cleaves the nonstructural polyprotein precursors. It may also be involved in shutoff of minus-strand RNA synthesis. nsP4 appears to function as the viral polymerase or elongation factor. The functions of nsP3 are as yet unresolved

Light front field theory of relativistic quark matter

Light-front quantization to many-particle systems of finite temperature and density provides a novel approach towards a relativistic description of quark matter and allows us to calculate the perturbative as well as the non-perturbative regime of QCD. Utilizing a Dyson expansion of light-front many-body Green functions we have so far calculated three-quark, quark-quark, and quark-antiquark correlations that lead to the chiral phase transition, the formation of hadrons and color superconductivity in a hot and/or dense environment. Presently, we use an effective zero-range interaction, to compare our results with the more traditional instant form approach where applicable.Comment: contribution to Quark Matter 2005, 18th International Conference on Nucleus Nucleus Colisions, 4 pages, 2 figures, hiph-preprint.sty file neede

The Scientist-Practitioner in a Counselling Psychology Setting

The human psyche is influenced by an extraordinary complexity of experiences. Many would therefore maintain that we can never completely understand another human being. As scientist-practitioners, is our purported allegiance to, and reliance upon, ‘official’ sources of knowledge (including theory and scientific evidence) sufficient for us to be confident that we can construct consistently helpful solutions from the myriad clinical data at our fingertips? Should we as psychologists accept that full understanding of causality is simply not an achievable objective? If we adopt the position that we can never fully explain causes, however, what role do we actually play? Can our interventions even be considered valid, let alone scientific? The question of how practitioners reflect upon their activity, and of the scientific assumptions behind their work, has occupied much debate in the field of psychology, and the many different strands of this debate are woven throughout the fabric of this book. In this chapter, we consider some of the many implications of this debate for counselling psychologists. Specifically, we begin by exploring the position of counselling psychology within the profession more broadly, and consider its place in the current controversy about the scientist-practitioner role. Next, we articulate some of our own practice in this regard, attempting not only to make note of the systematic approaches that we employ in counselling psychology but also to incorporate the wide range of expectation and experience that comes to the therapeutic endeavour. Finally, we try to define the type of scientist-practitioner that we envision in a counselling psychology setting

Termination of psychotherapy: The journey of ten psychoanalytic and psychodynamic therapists

Objectives: Literature on termination originates mainly from clinical and theoretical accounts as well as practitioners' autobiographical reports. There is, however, a paucity of psychological research on termination, and studies of therapists' experiences are particularly rare. The purpose of this study is to examine the process of termination of therapy based on therapists' narratives of experiences of endings with patients. Design: Grounded Theory methodology has been applied in this study in order to conceptualise the process of termination from the therapist’s perspective. Methods: Ten psychoanalytic and psychodynamic therapists were interviewed for this study. Results: Grounded Theory analysis of the data revealed five central categories: therapist as a person, therapist's awareness of termination, development of therapeutic relationship, working through termination, and the aftermath (post-termination phase). Conclusions: The results offer a Grounded Theory model of the therapist's journey through termination of therapy with patients. Subcategories and their relationships will be explored. Implications for clinical practice, limitations and suggestions for further research will be discussed

Site-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoprotease

The structural proteins of Sindbis virus are translated as a polyprotein precursor that is cleaved upon translation. The capsid protein is postulated to be a serine protease that releases itself from the N terminus of the nascent polyprotein by autoproteolysis. We have tested the importance in autoproteolysis of His-141, Asp-147, and Ser-215, previously postulated to form the catalytic triad of the protease, and of Asp-163. Several site-specific mutations were constructed at each of these positions, and the release of the capsid protein during translation in a cell-free system was examined. Because proteolysis occurs in cis during translation, the kinetics of release cannot be determined in this system, but the extent of proteolysis can be ascertained. Ser-215 appears to be the catalytic serine of the proteinase. Cys or Thr could substitute inefficiently for Ser-215, but substitution with Ala or Ile led to complete loss of activity. His-141 was also important for proteolysis. Substitution with Ala or Pro led to total loss of activity. Surprisingly, substitution with Arg resulted in complete proteolysis in vitro. Changes at the two Asp residues resulted in complete proteolysis of the substrate in vitro. All mutations that resulted in at least partial cleavage in vitro were incorporated into a full-length clone of Sindbis virus and an attempt was made to recover mutant virus. All changes tested were lethal for the virus except Asp-163 to Asn. Thus, production of infectious virus is either a more sensitive measure of the catalytic rate than the extent of in vitro cleavage, or these residues have necessary functions in addition to their possible role in proteolysis